全文获取类型
收费全文 | 9320篇 |
免费 | 959篇 |
国内免费 | 30篇 |
专业分类
耳鼻咽喉 | 165篇 |
儿科学 | 381篇 |
妇产科学 | 203篇 |
基础医学 | 1249篇 |
口腔科学 | 97篇 |
临床医学 | 940篇 |
内科学 | 1589篇 |
皮肤病学 | 357篇 |
神经病学 | 1008篇 |
特种医学 | 307篇 |
外科学 | 1260篇 |
综合类 | 241篇 |
一般理论 | 5篇 |
预防医学 | 1216篇 |
眼科学 | 134篇 |
药学 | 669篇 |
中国医学 | 4篇 |
肿瘤学 | 484篇 |
出版年
2021年 | 94篇 |
2020年 | 68篇 |
2019年 | 112篇 |
2018年 | 133篇 |
2017年 | 124篇 |
2016年 | 94篇 |
2015年 | 116篇 |
2014年 | 191篇 |
2013年 | 295篇 |
2012年 | 326篇 |
2011年 | 364篇 |
2010年 | 214篇 |
2009年 | 209篇 |
2008年 | 393篇 |
2007年 | 494篇 |
2006年 | 412篇 |
2005年 | 367篇 |
2004年 | 369篇 |
2003年 | 359篇 |
2002年 | 347篇 |
2001年 | 343篇 |
2000年 | 405篇 |
1999年 | 309篇 |
1998年 | 147篇 |
1997年 | 92篇 |
1996年 | 113篇 |
1995年 | 109篇 |
1994年 | 111篇 |
1993年 | 101篇 |
1992年 | 271篇 |
1991年 | 220篇 |
1990年 | 254篇 |
1989年 | 218篇 |
1988年 | 211篇 |
1987年 | 199篇 |
1986年 | 188篇 |
1985年 | 211篇 |
1984年 | 134篇 |
1983年 | 93篇 |
1982年 | 103篇 |
1981年 | 78篇 |
1980年 | 76篇 |
1979年 | 116篇 |
1978年 | 100篇 |
1977年 | 83篇 |
1976年 | 88篇 |
1975年 | 82篇 |
1974年 | 93篇 |
1973年 | 94篇 |
1972年 | 74篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
62.
Clostridium perfringens enterotoxin C‐terminal domain labeled to fluorescent dyes for in vivo visualization of micrometastatic chemotherapy‐resistant ovarian cancer 下载免费PDF全文
Emiliano Cocco Erik M. Shapiro Sara Gasparrini Salvatore Lopez Carlton L. Schwab Stefania Bellone Ileana Bortolomai Natalia J. Sumi Elena Bonazzoli Roberta Nicoletti Yang Deng W. Mark Saltzman Caroline J. Zeiss Floriana Centritto Jonathan D. Black Dan‐Arin Silasi Elena Ratner Masoud Azodi Thomas J. Rutherford Peter E. Schwartz Sergio Pecorelli Alessandro D. Santin 《International journal of cancer. Journal international du cancer》2015,137(11):2618-2629
Identification of micrometastatic disease at the time of surgery remains extremely challenging in ovarian cancer patients. We used fluorescence microscopy, an in vivo imaging system and a fluorescence stereo microscope to evaluate fluorescence distribution in Claudin‐3‐ and ‐4‐overexpressing ovarian tumors, floating tumor clumps isolated from ascites and healthy organs. To do so, mice harboring chemotherapy‐naïve and chemotherapy‐resistant human ovarian cancer xenografts or patient‐derived xenografts (PDXs) were treated with the carboxyl‐terminal binding domain of the Clostridium perfringens enterotoxin (c‐CPE) conjugated to FITC (FITC‐c‐CPE) or the near‐infrared (NIR) fluorescent tag IRDye CW800 (CW800‐c‐CPE) either intraperitoneally (IP) or intravenously (IV). We found tumor fluorescence to plateau at 30 min after IP injection of both the FITC‐c‐CPE and the CW800‐c‐CPE peptides and to be significantly higher than in healthy organs (p < 0.01). After IV injection of CW800‐c‐CPE, tumor fluorescence plateaued at 6 hr while the most favorable tumor‐to‐background fluorescence ratio (TBR) was found at 48 hr in both mouse models. Importantly, fluorescent c‐CPE was highly sensitive for the in vivo visualization of peritoneal micrometastatic tumor implants and the identification of ovarian tumor spheroids floating in malignant ascites that were otherwise not detectable by conventional visual observation. The use of the fluorescent c‐CPE peptide may represent a novel and effective optical approach at the time of primary debulking surgery for the real‐time detection of micrometastatic ovarian disease overexpressing the Claudin‐3 and ‐4 receptors or the identification of residual disease at the time of interval debulking surgery after neoadjuvant chemotherapy treatment. 相似文献
63.
Clinico‐pathological factors influencing lymph node yield in colorectal cancer and impact on survival: Analysis of new zealand cancer registry data 下载免费PDF全文
64.
65.
Rong Liu Stephanie Curry Patricia McMonagle Wendy W. Yeh Steven W. Ludmerer Patricia A. Jumes William L. Marshall Stephanie Kong Paul Ingravallo Stuart Black Irene Pak Mark J. DiNubile Anita Y. M. Howe 《Antimicrobial agents and chemotherapy》2015,59(11):6922-6929
Elbasvir is an investigational NS5A inhibitor with in vitro activity against multiple HCV genotypes. Antiviral activity of elbasvir was measured in replicons derived from wild-type or resistant variants of genotypes 1a, 1b, and 3. The barrier to resistance was assessed by the number of resistant colonies selected by exposure to various elbasvir concentrations. In a phase 1b dose-escalating study, virologic responses were determined in 48 noncirrhotic adult men with chronic genotype 1 or 3 infections randomized to placebo or elbasvir from 5 to 50 mg (genotype 1) or 10 to 100 mg (genotype 3) once daily for 5 days. The NS5A gene was sequenced from plasma specimens obtained before, during, and after treatment. Elbasvir suppressed the emergence of resistance-associated variants (RAVs) in vitro in a dose-dependent manner. Variants selected by exposure to high elbasvir concentrations typically encoded multiple amino acid substitutions (most commonly involving loci 30, 31, and 93), conferring high-level elbasvir resistance. In the monotherapy study, patients with genotype 1b had greater reductions in HCV RNA levels than patients with genotype 1a at all elbasvir doses; responses in patients with genotype 3 were generally less pronounced than for genotype 1, particularly at lower elbasvir doses. M28T, Q30R, L31V, and Y93H in genotype 1a, L31V and Y93H in genotype 1b, and A30K, L31F, and Y93H in genotype 3 were the predominant RAVs selected by elbasvir monotherapy. Virologic findings in patients were consistent with the preclinical observations. NS5A-RAVs emerged most often at amino acid positions 28, 30, 31, and 93 in both the laboratory and clinical trial. (The MK-8742 P002 trial has been registered at ClinicalTrials.gov under identifier .) NCT01532973相似文献
66.
Mitchell G. Thompson Vu Truong-Le Yonas A. Alamneh Chad C. Black Jeff Anderl Cary L. Honnold Rebecca L. Pavlicek Rania Abu-Taleb Matthew C. Wise Eric R. Hall Eric J. Wagar Eric Patzer Daniel V. Zurawski 《Antimicrobial agents and chemotherapy》2015,59(10):6484-6493
Skin and soft tissue infections (SSTIs) are a common occurrence in health care facilities with a heightened risk for immunocompromised patients. Klebsiella pneumoniae has been increasingly implicated as the bacterial agent responsible for SSTIs, and treatment can be challenging as more strains become multidrug resistant (MDR). Therefore, new treatments are needed to counter this bacterial pathogen. Gallium complexes exhibit antimicrobial activity and are currently being evaluated as potential treatment for bacterial infections. In this study, we tested a topical formulation containing gallium citrate (GaCi) for the treatment of wounds infected with K. pneumoniae. First, the MIC against K. pneumoniae ranged from 0.125 to 2.0 μg/ml GaCi. After this in vitro efficacy was established, two topical formulations with GaCi (0.1% [wt/vol] and 0.3% [wt/vol]) were tested in a murine wound model of MDR K. pneumoniae infection. Gross pathology and histopathology revealed K. pneumoniae-infected wounds appeared to close faster with GaCi treatment and were accompanied by reduced inflammation compared to those of untreated controls. Similarly, quantitative indications of infection remediation, such as reduced weight loss and wound area, suggested that treatment improved outcomes compared to those of untreated controls. Bacterial burdens were measured 1 and 3 days following inoculation, and a 0.5 to 1.5 log reduction of CFU was observed. Lastly, upon scanning electron microscopy analysis, GaCi treatment appeared to prevent biofilm formation on dressings compared to those of untreated controls. These results suggest that with more preclinical testing, a topical application of GaCi may be a promising alternative treatment strategy for K. pneumoniae SSTI. 相似文献
67.
Marita Falkmer Melissa Black Julia Tang Patrick Fitzgerald Sonya Girdler Denise Leung 《Developmental neurorehabilitation》2016,19(2):117-122
Objective: While local bias in visual processing in children with autism spectrum disorders (ASD) has been reported to result in difficulties in recognizing faces and facially expressed emotions, but superior ability in disembedding figures, associations between these abilities within a group of children with and without ASD have not been explored. Methods: Possible associations in performance on the Visual Perception Skills Figure–Ground test, a face recognition test and an emotion recognition test were investigated within 25 8–12-years-old children with high-functioning autism/Asperger syndrome, and in comparison to 33 typically developing children. Results: Analyses indicated a weak positive correlation between accuracy in Figure–Ground recognition and emotion recognition. No other correlation estimates were significant. Conclusion: These findings challenge both the enhanced perceptual function hypothesis and the weak central coherence hypothesis, and accentuate the importance of further scrutinizing the existance and nature of local visual bias in ASD. 相似文献
68.
Development of White Matter Hyperintensity Is Preceded by Reduced Cerebrovascular Reactivity 下载免费PDF全文
69.
Paulo Roberto Lima Falco do Vale Sheila Cerqueira Hudson P. Santos Beth P. Black Evanilda Souza de Santana Carvalho 《Nursing inquiry》2019,26(1)
The rapidly increasing number of cases of Zika virus and limited understanding of its congenital sequelae (e.g., microcephaly) led to stories of fear and uncertainty across social media and other mass communication networks. In this study, we used techniques generic to netnography, a form of ethnography, using Internet‐based computer‐mediated communications as a source of data to understand the experience and perceptions of families with infants diagnosed with Zika‐related microcephaly. We screened 27 YouTube? videos published online between October 2015 and July 2016, during which the Zika epidemic started, peaked, and declined. We identified three themes: (a) experiencing the news of a diagnosis of Zika‐associated microcephaly; (b) experiencing feelings and expectations of the ‘imperfect’ child; and (c) seeking to understand microcephaly to care for the child. We found that families experienced distressing feelings of shock, sadness, hopelessness, and pain, while dealing with emerging and sometimes conflicting information being transmitted by news outlets, uncertainty about the child's health, and healthcare providers’ lack of clarity to guide the family members. The ‘unknown’ factor of ZIKA was an additional stressful factor in the experience of the families. 相似文献
70.